Using Heterologous COS-7 Cells to Identify Semaphorin-Signaling Components.

Doci, Colleen L.; Gutkind, J. Silvio; Sakurai, Atsuko

doi:10.1007/978-1-4939-6448-2_11

MLA citation style (9th ed.)

Doci, Colleen L, Gutkind, J. Silvio, and Sakurai, Atsuko. Using Heterologous Cos-7 Cells to Identify Semaphorin-signaling Components. Springer. 2017. marian.palni-palci-staging.notch8.cloud/concern/generic_works/7fd5a752-508b-4496-9fb2-1cb53495911d?locale=de.

APA citation style (7th ed.)

D. C. L, G. J. Silvio, & S. Atsuko. (2017). Using Heterologous COS-7 Cells to Identify Semaphorin-Signaling Components. https://marian.palni-palci-staging.notch8.cloud/concern/generic_works/7fd5a752-508b-4496-9fb2-1cb53495911d?locale=de

Chicago citation style (CMOS 17, author-date)

Doci, Colleen L., Gutkind, J. Silvio, and Sakurai, Atsuko. Using Heterologous Cos-7 Cells to Identify Semaphorin-Signaling Components. Springer. 2017. https://marian.palni-palci-staging.notch8.cloud/concern/generic_works/7fd5a752-508b-4496-9fb2-1cb53495911d?locale=de.

Note: These citations are programmatically generated and may be incomplete.

Semaphorins are a family of membrane-bound and secreted type of proteins which were initially identified as chemorepulsive axon guidance molecules. Plexins and neuropilins are two major receptor families of semaphorins, and their common downstream targets are the actin cytoskeleton and cell-to-extracellular matrix adhesions. Semaphorins promote the collapse of growth cones by inducing rapid changes in the cytoskeleton and disassembly of focal adhesion structures. When transfected with appropriate receptors, non-neuronal COS-7 cells exhibit a similar cell collapse phenotype upon semaphorin stimulation. This heterologous system using COS-7 cells has been developed and widely used to investigate semaphorin-signaling pathways. In this chapter, we describe a COS-7 collapse assay protocol used to identify semaphorin-signaling components and a method to produce recombinant class 3 semaphorin proteins.

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