Confirmation of the Presence of Antibiotic Resistance Gram-Negative Bacteria in the Nina Mason Pulliam EcoLab (NMPE) by Kirby Bauer Assays and Genome Sequencing

Antibiotic resistant (AR) bacteria pose a serious threat to an individual’s health, and the presence of AR genes can lead to the development of “superbugs” that are resistant to currently used antibiotics. Identifying local reservoirs that house AR bacterial strains has become critical. The main objective of our project was to identify the presence and cause of bacterial resistance in gram-negative bacteria collected from Jensen Lake at the Nina Mason Pulliam Ecolab (NMPE). This research was conducted via two protocols. To test for AR, gram-negative bacteria isolated from the NMPE water samples were subjected to Kirby-Bauer assays (Fig. 1). To test for the presence of AR genes, genomic DNA was purified from the bacterial isolates and sent to an outside vendor (ID Genomics) for identification of potential AR genes through PCR amplification and sequencing (Fig. 2).

Creator

• Malhi, Jaipal
• Lex, Jake
• Hodges, Angela
• Khan, Z.
• Francois, Chaina
• Akbar, Samina

Contributeurs

• Purdue University

Language

• English

Mot-clé

• Antibiotic resistant (AR) bacteria
• gram-negative bacteria
• Jensen Lake
• Nina Mason Pulliam Ecolab (NMPE)
• Kirby-Bauer assay
• PCR amplification and sequencing

Date created

• 2023

Resource type

• Poster

Rights statement

• http://rightsstatements.org/vocab/InC/1.0/