MU-COM Research Day
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Last Updated: 2021-10-22
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MU-COM Research Day is an annual showcase of the the research engagement of the students within the College of Osteopathic Medicine. Content on display are typically poster presentations along with faculty-led discussions of research projects. This event is coordinated by the MU-COM Research Club as well as the Faculty-led Research Club Committee.- Articulos totales
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265
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Trabajos (262)
231.Short Duration Exposure of Vibrio Cholerae to Small Intestine Conditions and the Effect on Cholera Toxin and Stringent Response Gene Expression
- Title Tesim:
- Short Duration Exposure of Vibrio Cholerae to Small Intestine Conditions and the Effect on Cholera Toxin and Stringent Response Gene Expression
- Creador:
- Raskin, David and Stewart, Michael
- Descripción:
- Context: Vibrio cholerae is the causative agent of cholera, the epidemic diarrheal disease. The colonization and subsequent diarrheal symptoms are produced due to toxin coregulated pilus (TCP) and cholerae toxin (CT) respectively. Regulation of their expression involves a complex regulon, toxR, which is composed of toxT, a transcription factor that directly initiates transcription of the ct and tcp genes; and its upstream regulators tcpP and toxT, which require transcription factors tcpH and toxS. Objective: Most research in V. cholerae to date has shown expression of these virulence factors after a long exposure to small intestine conditions. The goal of this work was to determine changes that happen immediately after V. cholerae is exposed to conditions found in the small intestine. Design: Experimental Setting: MUCOM biologic research lab Methods: Deletions were made in regulon genes toxR, tcpPH, and toxT, as well as the stringent response regulators relA, spoT and relV. Expression of CT and TCP were quantified via the expression of the first gene in the ctx and tcp operons using real time PCR. Results: In tcpPH knock outs, there was still expression of TCP, similar to that of wild type. Moreover, with the loss of toxT, tcpPH, toxR, or a combination of toxR/toxT, toxR/tcpPH expression levels of CT were either absent or very minimal. Conclusions: These results show differences from those in which these strains are present in small intestine conditions for longer periods of time, particularly for the stringent response null strains. This may indicate that initial adjustment to the small intestine is affected by stringent response activity, but longer term expression of virulence factors do not require stringent response but only the toxR regulon. Further investigation will be required for understanding how V. cholerae use environmental signals to regulate virulence gene expression.
- Declaración de derechos:
- http://rightsstatements.org/vocab/InC/1.0/
- Idioma:
- English
- Tipo de recurso:
- poster
- Palabra clave:
- cholerae toxin (CT), Vibrio cholerae, and toxin coregulated pilus (TCP)
- Fecha de Creacion:
- 11/10/2017
232.Should patients with systemic lupus erythematosus (SLE) RECEIVE a heart transplant?
- Title Tesim:
- Should patients with systemic lupus erythematosus (SLE) RECEIVE a heart transplant?
- Creador:
- Zafar, Nayab, Sehgal, Neha, and Kelly, K.
- Descripción:
- This was a case study on a patient with SLE who received a heart transplant. Patients with SLE are generally not considered to be ideal candidates for transplantation due to auto-immunity involving many organ systems. Historically, patients with SLE requiring kidney transplantation have had good prognosis, but there is a weaker consensus sur-rounding heart transplantation in patients with SLE-related cardiomyopathy and heart failure. A 24-year-old female patient presented in 2016 with cardiogenic shock with a history of non-ischemic dilated cardiomyopathy with an ejection fraction of 5-10% and SLE com-plicated by nephritis. She required 2-3 hospital visits per year due to heart failure and SLE complications. A chest X-ray revealed cardiomegaly with hypo-inflated lungs. She was placed on inotropic support and followed up with a heart transplant in 2017. During transplantation, her heart was found to have dense adhesions resulting from SLE. In 2018, the patient returned with acute kidney injury and chronic kidney disease (CKD) Stage 3 with metabolic acidosis and a diastolic dysfunction. Her chest X-ray presented with mild cardiomegaly and a kidney ultrasound was negative for hydronephrosis. She was diagnosed with NYHA class III heart failure and was prescribed torsemide and a therapeutic plan to begin her on allopurinol due to her reduced GFR. After immunosup-pressive therapy, her Stage IV lupus nephritis was not active at the time of her dis-charge. Conclusion: This case demonstrates the ongoing challenges that physicians encoun-ter when managing patients with SLE-related cardiac involvement. The literature cites no clear consensus regarding management of patients with cardiac involvement of SLE, especially considering the large range of cardiac involvement. This case supports the growing need for understanding and managing cardiac involvement in patients with SLE.
- Declaración de derechos:
- http://rightsstatements.org/vocab/InC/1.0/
- Idioma:
- English
- Tipo de recurso:
- poster
- Fecha de Creacion:
- 11/22/2019
233.Single-Center Denial Reasons for Potential Living Kidney Donors
- Title Tesim:
- Single-Center Denial Reasons for Potential Living Kidney Donors
- Creador:
- Lien, Savahanna, Matas, Arthur, Voges, Margaret, and Garvey, Cathy
- Descripción:
- Because of the benefits of preemptive living donor (LD) transplant, the desire for LD is rising. However, in the last decade, there has been no increase in LD in the U.S, possibility due to older donor candidate population leading to increased denial rates. We previously studied denial rates and cause for denial for donor candidates between 2009 and 2011. We herein present for comparison causes for denial for donor candidates between 8/2012 and 6/2015. During the interval, our acceptance criteria have not changed. Results: Between August 2012 and June 2015, we evaluated 644 potential living donors: 2012=88, 2013=222, 2014=220, 2015 (Jan-June) =114. Of these, 31% of candidates were denied: 26% for medical reasons, 5% for psychosocial reasons. Mean age of approved candidates = 41 (range: 18-75); average denied = 43 (range, 18-72). To compare, between 2009-2011, 36% candidates were denied (32% medical, 4% psychosocial). The mean age for approved candidates = 40, while denied candidates mean age = 47 (range, 18-64). Current candidates most common medical denials reasons were obesity and suboptimal kidney anatomy, although the distribution of reasons varies by age cohort. Historical group (2009-2011): most common medical denials were hypertension and poor kidney function. Psychosocial denial reasons included: mental illness, lack of support and substance abuse. Conclusion: Over an interval of 6.5 years, the rate of donor candidate denial at our center has not changed. Overall, there has been a change in major reasons for denial; importantly, reasons for denial vary by donor age.
- Declaración de derechos:
- http://rightsstatements.org/vocab/InC/1.0/
- Idioma:
- English
- Tipo de recurso:
- poster
- Palabra clave:
- living donor (LD), denial reasons
- Fecha de Creacion:
- 1/1/2015
234.Sorbitol Protection Assay Provides Insight into Antifungal Mechanisms of Boron Compounds against Candida albicans and Candida Glabrata
- Title Tesim:
- Sorbitol Protection Assay Provides Insight into Antifungal Mechanisms of Boron Compounds against Candida albicans and Candida Glabrata
- Creador:
- Larsen, Bryan
- Descripción:
- In order to determine if loss of cell wall integrity contributes to boric acid toxicity towards wild type and drug resistant Candida organisms, yeast growth in osmoprotective-high-sorbitol medium was compared to growth determined by turbidity (A<sub>450</sub>) in sorbitol-free medium. Initially it was discovered that hyperosmolarity without added boron (sorbitol medium vs Sabouraud’s dextrose medium) reduced turbidity by an average of 42% for 27 yeast strains. The addition of 0.2% boric acid to this test resulted in sorbitol protection equal to an average 6.5-fold increase in turbidity in the presence of sorbitol. We also determined that organoboron compounds obtained from commercial nutritional supplement products also inhibited growth of Candida. Based on this finding we applied the sorbitol protection assay to 16 selected strains of Candida with the result that sorbitol protection was also seen with boron gluconate.
- Declaración de derechos:
- http://rightsstatements.org/vocab/InC/1.0/
- Idioma:
- English
- Tipo de recurso:
- poster
- Palabra clave:
- cell wall integrity, yeast growth in osmoprotective-high-sorbitol medium, and boric acid toxicity
- Fecha de Creacion:
- 11/11/2017
235.Spontaneous resolution of fetal ascites secondary to gastrointestinal abnormality
- Title Tesim:
- Spontaneous resolution of fetal ascites secondary to gastrointestinal abnormality
- Creador:
- Yannetti, Melissa, Wiles, A, and Dinglas, C
- Related Url Tesim:
- Audio presentation: https://marian.hosted.panopto.com/Panopto/Pages/Viewer.aspx?id=6beb4b6a-ad70-4873-b5cd-ac7400301803
- Descripción:
- Reported cases of isolated fetal ascites determined to be idiopathic have been shown to carry a favorable prognosis with frequent spontaneous resolution in utero (1,2,3). These cases generally reported better fetal outcomes with delivery of normal neonates as compared to cases with associated conditions such as gastrointestinal and genitourinary abnormalities, intrauterine infections, and overlapping cases with hydrops (1). No existing reports of antenatally resolving fetal ascites with a discovered gastrointestinal anomaly describe concurrent resolution of the underlying abnormality without surgical correction or postpartum regression with medical management In this case, fetal ascites was prenatally diagnosed on ultrasound at 32 weeks secondary to a suspected gastrointestinal abnormality and was found to have spontaneously resolved in utero with a normal neonatal course.
- Declaración de derechos:
- http://rightsstatements.org/vocab/InC/1.0/
- Idioma:
- English
- Tipo de recurso:
- Poster
- Palabra clave:
- gastrointestinal and genitourinary abnormalities and fetal ascites
- Fecha de Creacion:
- 2020
236.Stolen ambulances reported in the media
- Title Tesim:
- Stolen ambulances reported in the media
- Creador:
- Sullivan, Ryan and Kraus, C
- Related Url Tesim:
- Audio presentation: https://marian.hosted.panopto.com/Panopto/Pages/Viewer.aspx?id=f4a9c189-b4b3-4f87-8a00-ac74012caf47
- Descripción:
- Ambulances are a target for theft and reportings of ambulance thefts has been increasing in the past several years. There is a paucity of research in this area, as a PubMed search of the terms "ambulance theft" yields only one relevant article: Alves OW, Bissel RA. Ambulance Snatching: How vulnerable are we? J Emerg Med. 2003; 25(2):211-214.
- Declaración de derechos:
- http://rightsstatements.org/vocab/InC/1.0/
- Idioma:
- English
- Tipo de recurso:
- Poster
- Palabra clave:
- ambulance theft
- Fecha de Creacion:
- 2020
237.Stopping Pandemics in their Tracks
- Title Tesim:
- Stopping Pandemics in their Tracks
- Creador:
- Mulye, Minal, Schmid, Aaron, Khat, Israt, Abraham, Benjamin, and Akbar, Samina
- Related Url Tesim:
- Video Presentation: https://marian.hosted.panopto.com/Panopto/Pages/Viewer.aspx?id=fcc763dc-5ad7-43e2-8624-ac730005b5d1
- Descripción:
- The proposed solution is a self-reported, symptoms-based syndromic surveillance system that is universal, interactive, integrative, and combined with artificial intelligence. Once developed, this framework has the potential to stop any future epidemics and pandemic in urban and rural areas worldwide. We conducted a thorough literature review of existing short message service (SMS, text messaging) and interactive voice response (IVR, calling) surveillance systems, identified and addressed the shortcomings. We have concluded that many of the previous syndromic surveillance models suffer from data fragmentation, thus hindering scalability their to a global setting. This proposal will allow decision-making officials and healthcare professionals to robustly identify local disease outbreaks, thus thwarting unchecked spread while preventing a breakdown in the supply chain. Automation of healthcare team reported findings will enable predictive diagnosis and prognosis, thereby allocating medical resources appropriately. Disease-specific databases will benefit from real-time refinement, then utilizable for prospective case mitigation, medical management, and pathogen containment.
- Declaración de derechos:
- http://rightsstatements.org/vocab/InC/1.0/
- Idioma:
- English
- Tipo de recurso:
- Poster
- Palabra clave:
- syndromic surveillance system, artificial intelligence, epidemics, and pandemics
- Fecha de Creacion:
- 2020
238.Stringent Response Regulation of Virulence Factors in Vibrio Cholera
- Title Tesim:
- Stringent Response Regulation of Virulence Factors in Vibrio Cholera
- Creador:
- Raskin, David, Cooper, Jennifer, Mishra, Arunima, He, Huajun, and Mall, Swathi
- Descripción:
- Vibrio cholerae colonization of the host requires stringent response activation of TCP, the Toxin-Coregulated Pilus Cholera is an epidemic diarrheal disease caused by the Gramnegative bacterium Vibrio cholerae. In order to colonize a host, V. cholerae must express the toxin-coregulated pilus (TCP), and diarrheal symptoms are primarily produced by the virulence factor cholera toxin (CT). CT and TCP expression is regulated by the ToxR regulon. Components of the ToxR regulon include ToxT, a transcription factor that directly initiates transcription of the CT and TCP genes. Upstream regulators include the integral membrane proteins TcpP and ToxR, transcription factors necessary for expression of toxT. ToxR may also directly initiate transcription of the CT genes independently of ToxT. It is thought that TCP is required early in infection to colonize the small intestine and that CT is expressed later in order to produce diarrhea and escape the host. We found that stringent response, the universally-conserved low nutrient stress response, was necessary for colonization of the mouse small intestine. The hypothesis tested was that bacterial evaluation of nutrient environment regulated virulence genes through the stringent response control of the ToxR regulon. Deletions in the stringent response genes and of the ToxR regulon genes were made and these mutants used to determine where stringent response fit into the virulence gene regulatory system. Stringent response activated gene expression of virulence factors (those encoding CT and TCP) and some ToxR regulon components (toxT and tcpP) while it repressed others (toxR). The gene expression data was consistent with the requirement for stringent response-dependent colonization of the mouse; stringent response was required for TCP expression, with TCP required for colonization. The gene expression data showing that stringent response had differential effects on components in the ToxR regulon may explain how CT and TCP may be differentially regulated in infection despite being controlled by the same transcriptional activators.
- Declaración de derechos:
- http://rightsstatements.org/vocab/InC/1.0/
- Idioma:
- English
- Tipo de recurso:
- poster
- Palabra clave:
- Vibrio Cholera, toxin-coregulated pilus (TCP), cholera toxin (CT), and virulence factor
- Fecha de Creacion:
- 1/1/2016
239.Student Use of Virtual Microscopy Laboratory Modules in an Integrated Medical Curriculum
- Title Tesim:
- Student Use of Virtual Microscopy Laboratory Modules in an Integrated Medical Curriculum
- Creador:
- Chatterjee, Allison and Kotadia, Shirley
- Descripción:
- In conjunction with recent curricular changes at US medical schools, histology instruction has undergone significant changes. One major change has been the steady shift from traditional microscopy laboratory sessions to the integration of virtual microscopy programs. This shift has been matched by a decrease in total histology course contact hours, in part due to incorporation of histology into integrated medical curricula. Histology at MUCOM is fully integrated into a systems-based curriculum during the pre-clinical years. Additionally, self-directed virtual histology laboratory modules created in Digital Slidebox take the place of formal microscopy laboratory sessions. These modules were designed to be interactive, collaborative, opennote/ open-book, and provide both formative and summative feedback via practice and graded quizzes respectfully. The purpose of this project was to determine if students utilize the self-directed virtual microscopy laboratory modules in the way they were designed to be used and explore any discrepancies. Two voluntary, anonymous Qualtrics surveys were used to collect data on how students use the virtual microscopy laboratory modules within the NEMS course during the Spring 2016 semester. 68 students responded to the first survey which focused on the first three histology lectures; 33 students responded to the second survey which focused on the last three histology lectures. Results indicated that students do favor group study with more students working in groups for the graded quizzes compared to the practice quizzes. Most students referred to the lecture PowerPoints while working on the quizzes; and about two-thirds of students used Google search. Surprisingly, about 80% of students prefer to take the practice quiz only once. Additionally, students who only occasionally worked in study groups found the practice quizzes more difficult than those who always or never worked in groups. Additional studies are planned to investigate patterns of use over time throughout the pre-clinical curriculum.
- Declaración de derechos:
- http://rightsstatements.org/vocab/InC/1.0/
- Idioma:
- English
- Tipo de recurso:
- poster
- Palabra clave:
- histology instruction, qualtrics, Digital Slidebox, and virtual microscopy
- Fecha de Creacion:
- 1/1/2016
240.Surgical Therapy to Atlantoaxial Instability
- Title Tesim:
- Surgical Therapy to Atlantoaxial Instability
- Creador:
- Cooke, Paul and Dufeau, David
- Related Url Tesim:
- Audio presentation: https://marian.hosted.panopto.com/Panopto/Pages/Viewer.aspx?id=f9d4d26f-284b-45fa-84b0-adf701233a01
- Descripción:
- Atlantoaxial instability (AAI) is a term that is used to describe instability at the atlantoaxial joint of the cervical spine. This is often from alar ligament instability causing the dens of the axis to be prone to move posterior or sublux. The inferior portion of the medulla oblongata and the superior portion of the spinal cord can be compressed by the posterior movement of the dens causing lifethreatening effects. In this presentation, a surgical therapy to atlantoaxial instability will be examined for the purpose of educating future medical students. We will specifically be examining AAI with spina-bifida occulta at C1
- Declaración de derechos:
- http://rightsstatements.org/vocab/InC/1.0/
- Idioma:
- English
- Tipo de recurso:
- Poster
- Palabra clave:
- Atlantoaxial Instability, spina-bifida occulta, and Surgical Therapy
- Fecha de Creacion:
- 2021