Faculty Publications and Research - MUCOM
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Last Updated: 2022-04-01
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1. 3D visualization in medical-student training
- Title Tesim:
- 3D visualization in medical-student training
- Schöpfer:
- Dufeau, David
- Related Url Tesim:
- https://repositories.lib.utexas.edu/bitstream/handle/2152/47194/Tosca%20Texas%20Booklet_FINAL%20No%20PRINT.pdf?sequence=3&isAllowed=y
- Beschreibung:
- Visualizing the spatial relationships of anatomical features, gross pathologies, and diagnostic findings is a fundamental part of the training of medical students and other learners in health-professions curricula. But to what extent can we augment the conventional training opportunities (e.g. Gross dissection, interpretation of sectional imagery, interpretation of histological sections) with 3D enhanced visualizations of anatomic, histologic, and diagnostic data-sets? Here we present a case study of medical-students at Marian University College of Osteopathic Medicine utilizing 3D visualization and printing techniques as part of a summer training opportunity. Medical students, in the summer between their first and second year of medical school training, self-identified an interest in the interpretation of sectional imagery. These students were then encouraged to design a project with the aim of presenting a 3D visualization of an anatomic or pathologic study that could be better understood in a 3D format than conventional imaging formats. The students also identified the target audience for these studies— these included student-doctors, medical residents, and clinical patients. Case studies the students completed included visualizations of maxillofacial surgical interventions, pediatric cardiac defects, neurological tracts, cerebral basal ganglia, and paranasal sinuses, among others. The resulting 3D interpretations were then presented as either 3D prints (utilizing stereolithography), YouTube videos, interactive 3D PDF files, or some combination of these media. It is possible to develop case studies to a high degree of maturity during a summer program. The next step in this study is to identify the efficacy of these presentations in various learning environments.
- Erklärung der Rechte:
- http://rightsstatements.org/vocab/InC/1.0/
- Sprache:
- English
- Ressourcentyp:
- Conference
- Stichwort:
- medical education diagnostic imaging, 3D visualization, 3D printing, stereolithography, histology, and sectional imagery
- Datum erstellt:
- 6/6/2017
2. A Murine Model of Waning Scrub Typhus Cross-Protection between Heterologous Strains of Orientia tsutsugamushi
- Title Tesim:
- A Murine Model of Waning Scrub Typhus Cross-Protection between Heterologous Strains of Orientia tsutsugamushi
- Schöpfer:
- Xu, Guang, Mendell, N., Bouyer, D., Walker, D., and Shelite, T.
- Related Url Tesim:
- Available from the publisher: https://www.mdpi.com/2076-0817/11/5/512/htm and Available from the library catalog: https://marianunivindianapolis.on.worldcat.org/oclc/9514689777
- Beschreibung:
- Orientia tsutsugamushi, the etiologic agent of the life-threatening febrile disease scrub typhus, is an obligately intracellular small coccobacillary bacterium belonging to the family Rickettsiaceae and is transmitted by the parasitic larval stage of trombiculid mites. Progress towards a vaccine for protection against scrub typhus has been impeded by characteristics of the pathogen and the infection. There are numerous strains of O. tsutsugamushi in the Asia-Pacific region with geographical overlap. In human cases immunity has been described as poor against heterologous strains of the pathogen, as well as short-lived against the homologous strain, with a mean antibody reversion rate of less than one year. Animal models of cross-protection as well as of deterioration of this cross-protection are needed to enhance understanding of transient immunity to scrub typhus. To build upon current understanding of this ineffective protection we sought to utilize our recently developed models, sublethal intradermal infection followed by challenge via ordinarily lethal hematogenous dissemination. Mice that were initially infected sublethally with O. tsutsugamushi Gilliam strain and were challenged with an ordinarily lethal dose of heterologous Karp strain were protected from death by a robust immune response at one month after the primary infection as evidenced by an abundance of mononuclear cellular infiltrates in target organs such as lung, liver, and kidney; maintenance of body weight; and low bacterial loads in the organs. Waning protection from lethal Karp strain challenge indicated by weight loss mirroring that observed in naïve mice was observed as early as 9 months after primary Gilliam strain infection, and higher bacterial loads, severe disease, and eventual death in some mice was observed after challenge with Karp strain at 14 months post-initial heterologous infection.
- Erklärung der Rechte:
- http://rightsstatements.org/vocab/InC/1.0/
- Lizenz:
- https://creativecommons.org/licenses/by/4.0/
- Sprache:
- English
- Herausgeber:
- MDPI
- Identifikator:
- DOI: https://doi.org/10.3390/pathogens11050512
- Ressourcentyp:
- Article
- Stichwort:
- hematogenous model, sublethal, scrub typhus, intradermal, cross-protection, immunity, and Orientia
- Datum erstellt:
- 2022
3. A unique variation of the jugular veins and its clinical significance
- Title Tesim:
- A unique variation of the jugular veins and its clinical significance
- Schöpfer:
- Young, McKenzie, Zavitsky, Alexis, Niceley, Jillian, Battiston, Gabriella, and Sakthi-Velavan, Sumathilatha
- Related Url Tesim:
- Available from the publisher: https://journals.viamedica.pl/folia_morphologica/article/view/98618, Available from the library catalog: https://marianunivindianapolis.on.worldcat.org/oclc/10160142074, and Available via PubMed: https://pubmed.ncbi.nlm.nih.gov/38390747/
- Beschreibung:
- The authors report a rare variation of the anterior jugular and internal jugular veins in a 78-year-old male donor. An enlarged and curved left anterior jugular vein (AJV) was formed as the continuation of the left common facial vein (CFV). The left AJV's diameter was wider than the internal jugular vein (IJV) and measured around 5 mm greater than the IJV's diameter and a channel connected the two veins. The right AJV and CFV continued from the two divisions of the right facial vein. The right AJV's diameter was smaller than the right IJV's diameter. The right external jugular vein was absent. No concurrent pathology supported the abnormal dimension of the left AJV and the findings were indicative of a variant anatomy. These variations have rarely been reported and have important clinical correlations. Failed IJV cannulation may result if the variant neck veins are missed. However, variant veins may serve as collateral channels and patch material in IJV reconstruction, carotid angioplasty, and ventricular-jugular shunts.
- Erklärung der Rechte:
- http://rightsstatements.org/vocab/InC/1.0/
- Lizenz:
- https://creativecommons.org/licenses/by/4.0/
- Sprache:
- English
- Herausgeber:
- Via Medica and Polish Anatomical Society
- Identifikator:
- PMID: 38390747 and DOI: 10.5603/fm.98618
- Ressourcentyp:
- Article
- Stichwort:
- anatomical variation, bilateral variation, catheterization, communicating vein, jugular veins, and venous anastomoses
- Datum erstellt:
- 2024
4. Abnormal Trafficking of Endogenously Expressed BMPR2 Mutant Allelic Products in Patients with Heritable Pulmonary Arterial Hypertension
- Title Tesim:
- Abnormal Trafficking of Endogenously Expressed BMPR2 Mutant Allelic Products in Patients with Heritable Pulmonary Arterial Hypertension
- Schöpfer:
- Frump, A.L., Hamid, R., de Caestecker, M., Austin, E.D., and Lowery, Jonathan W.
- Beschreibung:
- More than 200 heterozygous mutations in the type 2 BMP receptor gene, BMPR2, have been identified in patients with Heritable Pulmonary Arterial Hypertension (HPAH). More severe clinical outcomes occur in patients with BMPR2 mutations by-passing nonsense-mediated mRNA decay (NMD negative mutations). These comprise 40% of HPAH mutations and are predicted to express BMPR2 mutant products. However expression of endogenous NMD negative BMPR2 mutant products and their effect on protein trafficking and signaling function have never been described. Here, we characterize the expression and trafficking of an HPAH-associated NMD negative BMPR2 mutation that results in an in-frame deletion of BMPR2 EXON2 (BMPR2?Ex2) in HPAH patient-derived lymphocytes and in pulmonary endothelial cells (PECs) from mice carrying the same in-frame deletion of Exon 2 (Bmpr2 (?Ex2/+) mice). The endogenous BMPR2?Ex2 mutant product does not reach the cell surface and is retained in the endoplasmic reticulum. Moreover, chemical chaperones 4-PBA and TUDCA partially restore cell surface expression of Bmpr2?Ex2 in PECs, suggesting that the mutant product is mis-folded. We also show that PECs from Bmpr2 (?Ex2/+) mice have defects in the BMP-induced Smad1/5/8 and Id1 signaling axis, and that addition of chemical chaperones restores expression of the Smad1/5/8 target Id1. These data indicate that the endogenous NMD negative BMPR?Ex2 mutant product is expressed but has a folding defect resulting in ER retention. Partial correction of this folding defect and restoration of defective BMP signaling using chemical chaperones suggests that protein-folding agents could be used therapeutically in patients with these NMD negative BMPR2 mutations.
- Erklärung der Rechte:
- http://rightsstatements.org/vocab/InC/1.0/
- Sprache:
- English
- Identifikator:
- 10.1371/journal.pone.0080319
- Ressourcentyp:
- Article
- Stichwort:
- Bone Morphogenetic Protein Receptors, Alleles, Mutation, Nonsense Mediated mRNA Decay, Protein Transport, and Familial Primary Pulmonary Hypertension
- Datum erstellt:
- 1/1/2013
5. Addressing Motivations and Barriers to Research Involvement during Medical School among Osteopathic Medical Students in the United States
- Title Tesim:
- Addressing Motivations and Barriers to Research Involvement during Medical School among Osteopathic Medical Students in the United States
- Schöpfer:
- Zahl, Sarah, Hum, Julia M., Jackson, Krista L., Griffin, Nicole, Lowery, Jonathan W., Nick, Benjamin, Herber, Jake, Hamby, T., Baumann, Michael, and Ogunbekun, Oladipupo
- Related Url Tesim:
- Available from the publlisher: https://www.mdpi.com/2227-7102/12/6/407 and Available from the library catalog: https://marianunivindianapolis.on.worldcat.org/oclc/9532526413
- Beschreibung:
- Involvement in research is regarded as a high-impact educational practice, which, for medical professionals, is associated with sharpened critical thinking and life-long learning skills, greater appreciation for evidence-based medicine, and better clinical competence scores. However, there are limited data regarding the research experience and/or interest among osteopathic medical students in the United States despite a rapidly increasing enrollment and expansion of the number of osteopathic medical schools. Thus, we administered an electronic survey examining prior research experience, interests, and perceptions about research participation during medical school to four successive classes of incoming first-year osteopathic medical students. We also performed focus groups with rising third-year osteopathic medical students around the topic of perceived barriers to and potential enablers of promoting research participation. This yielded a survey addendum where first-year osteopathic medical students provided feedback on the likelihood of specific incentives/enablers to encourage participation in research during medical school. Overall, osteopathic medical students are interested in research, view research experience as valuable, and perceive research experience as beneficial to future career development. Students perceive that the primary barrier to involvement in research is a potential negative impact upon performance in coursework. Feedback on the likelihood of specific enablers/incentives was also garnered. Our findings from a single institution may have important implications in defining the prior experiences and perceptions held by first-year osteopathic medical students. Specifically, our study indicates that research experiences intentionally designed with (1) a strong likelihood of gaining a publication, (2) financial compensation, and (3) the opportunity for short-term involvement, a flexible time commitment, and/or a dedicated time period are most likely to encourage research participation by osteopathic medical students.
- Erklärung der Rechte:
- http://rightsstatements.org/vocab/InC/1.0/
- Lizenz:
- https://creativecommons.org/licenses/by/4.0/
- Sprache:
- English
- Herausgeber:
- MDPI
- Identifikator:
- DOI: https://doi.org/10.3390/educsci12060407
- Ressourcentyp:
- Article
- Stichwort:
- incentive, barrier, osteopathic medical school, undergraduate medical education, and focus groups
- Datum erstellt:
- 2022
6. Allele-specific RNA Interference in FOP Silencing the FOP Gene
- Title Tesim:
- Allele-specific RNA Interference in FOP Silencing the FOP Gene
- Schöpfer:
- Rosen, V. and Lowery, Jonathan W.
- Erklärung der Rechte:
- http://rightsstatements.org/vocab/InC/1.0/
- Sprache:
- English
- Identifikator:
- 10.1038/gt.2011.190
- Ressourcentyp:
- Article
- Stichwort:
- RNA, Mesenchymal Stromal Cells, Myositis Ossificans, Osteogenesis, Cell Differentiation, Activin Receptors, Type I, and Gene Silencing
- Datum erstellt:
- 7/1/2012
7. Am I big boned? Bone length scaled reference data for HRpQCT measures of the radial and tibial diaphysis in White adults
- Title Tesim:
- Am I big boned? Bone length scaled reference data for HRpQCT measures of the radial and tibial diaphysis in White adults
- Schöpfer:
- Warden, S., Fuchs, Robyn, Liu, Z., Toloday, K., Surowiec, R., and Moe, S.
- Related Url Tesim:
- Available from the publisher: https://www.sciencedirect.com/science/article/pii/S2352187224000020?via%3Dihub, Available from PubMed: https://pubmed.ncbi.nlm.nih.gov/38292934, and Available from the library catalog: https://marianunivindianapolis.on.worldcat.org/oclc/10192435007
- Beschreibung:
- Cross-sectional size of a long bone shaft influences its mechanical properties. We recently used high-resolution peripheral quantitative computed tomography (HRpQCT) to create reference data for size measures of the radial and tibial diaphyses. However, data did not take into account the impact of bone length. Human bone exhibits relatively isometric allometry whereby cross-sectional area increases proportionally with bone length. The consequence is that taller than average individuals will generally have larger z-scores for bone size outcomes when length is not considered. The goal of the current work was to develop a means of determining whether an individual's cross-sectional bone size is suitable for their bone length. HRpQCT scans performed at 30 % of bone length proximal from the distal end of the radius and tibia were acquired from 1034 White females (age = 18.0 to 85.3 y) and 392 White males (age = 18.4 to 83.6 y). Positive relationships were confirmed between bone length and cross-sectional areas and estimated mechanical properties. Scaling factors were calculated and used to scale HRpQCT outcomes to bone length. Centile curves were generated for both raw and bone length scaled HRpQCT data using the LMS approach. Excel-based calculators are provided to facilitate calculation of z-scores for both raw and bone length scaled HRpQCT outcomes. The raw z-scores indicate the magnitude that an individual's HRpQCT outcomes differ relative to expected sex- and age-specific values, with the scaled z-scores also considering bone length. The latter enables it to be determined whether an individual or population of interest has normal sized bones for their length, which may have implications for injury risk. In addition to providing a means of expressing HRpQCT bone size outcomes relative to bone length, the current study also provides centile curves for outcomes previously without reference data, including tissue mineral density and moments of inertia.
- Erklärung der Rechte:
- http://rightsstatements.org/vocab/InC/1.0/
- Lizenz:
- https://creativecommons.org/licenses/by/4.0/
- Sprache:
- English
- Herausgeber:
- Elsevier
- Identifikator:
- PMID: 38292934 , PMCID: PMC10824696 , and DOI: 10.1016/j.bonr.2024.101735
- Ressourcentyp:
- Article
- Stichwort:
- Bone allometry, Bone strength, Cortical bone, Normative data, and Osteoporosis
- Datum erstellt:
- 2024
8. Amyloid-beta Alzheimer targets - protein processing, lipid rafts, and amyloid-beta pores.
- Title Tesim:
- Amyloid-beta Alzheimer targets - protein processing, lipid rafts, and amyloid-beta pores.
- Schöpfer:
- Medhane, Cumbay, Arbor, Sage, and LaFontaine, Mike
- Beschreibung:
- Amyloid beta (A?), the hallmark of Alzheimer's Disease (AD), now appears to be deleterious in its low number aggregate form as opposed to the macroscopic A? fibers historically seen postmortem. While Alzheimer targets, such as the tau protein, amyloid precursor protein (APP) processing, and immune system activation continue to be investigated, the recent discovery that amyloid beta aggregates at lipid rafts and likely forms neurotoxic pores has led to a new paradigm regarding why past therapeutics may have failed and how to design the next round of compounds for clinical trials. An atomic resolution understanding of A? aggregates, which appear to exist in multiple conformations, is most desirable for future therapeutic development. The investigative difficulties, structures of these small A? aggregates, and current therapeutics are summarized in this review.
- Erklärung der Rechte:
- http://rightsstatements.org/vocab/InC/1.0/
- Sprache:
- English
- Ressourcentyp:
- Article
- Stichwort:
- Amyloid beta-Protein Precursor, Alzheimer Disease, Amyloid beta-Peptides, and Membrane Microdomains
- Datum erstellt:
- 3/24/2016
9. Anatomy, Abdomen and Pelvis, Internal Iliac Artery
- Title Tesim:
- Anatomy, Abdomen and Pelvis, Internal Iliac Artery
- Schöpfer:
- Samra, N., Zaunbrecher, N., and Arbor, Tafline
- Related Url Tesim:
- Available from the National Library of Medicine: https://www.ncbi.nlm.nih.gov/books/NBK537311/
- Beschreibung:
- The internal iliac artery (IIA), or hypogastric artery, is the primary artery supplying the pelvic viscera and an important contributor to structures of the pelvic wall, perineum, gluteal region, and thigh. The internal iliac artery arises where the common iliac artery bifurcates into internal and external iliac arteries; it then crosses the pelvic brim to give off numerous branches within the pelvis. The pelvic arteries arising from the internal iliac artery are highly variable in their branching pattern and number, an important feature to note during pelvic surgery. In addition, there are notable differences in the branches of the internal iliac artery in males and females, given that the reproductive organs are within their supply territory.
- Erklärung der Rechte:
- http://rightsstatements.org/vocab/InC/1.0/
- Lizenz:
- https://creativecommons.org/licenses/by/4.0/
- Sprache:
- English
- Herausgeber:
- StatPearls
- Identifikator:
- Bookshelf ID: NBK537311 and PMID: 30725996
- Stichwort:
- hypogastric artery and internal iliac artery (IIA)
- Datum erstellt:
- 2022
10. Bitter melon extract suppresses metastatic breast cancer cells (MCF-7 cells) growth possibly by hindering glucose uptake
- Title Tesim:
- Bitter melon extract suppresses metastatic breast cancer cells (MCF-7 cells) growth possibly by hindering glucose uptake
- Schöpfer:
- Kakuturu, Abhinav , Choi, Heeyun , Noe, Leah G., Scherer, Brianna N., Sharma, B., Khambu, B., and Bhetwal, Bhupal P.
- Related Url Tesim:
- Available via PubMed Central: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10509689/ and Available via the library catalog: https://marianunivindianapolis.on.worldcat.org/oclc/10015060304
- Beschreibung:
- Breast cancer is one of the most commonly diagnosed cancers among women, however the complete cure for metastatic breast cancer is lacking due to poor prognosis. There has been an increasing trend of dietary modifications including consumption of natural food for the prevention of cancer. One of the popular natural foods is bitter melon. Bitter melon grows in tropical and subtropical areas. Some of the beneficial effects of bitter melon towards disease including cancer have been reported at the whole body/organismal level. However, specific cellular mechanisms by which bitter melon exerts beneficial effects in breast cancer are lacking. In this study, we used a human metastatic breast cancer cell line, MCF-7 cell, to study if bitter melon alters glucose clearance from the culture medium. We co-cultured MCF-7 cells with bitter melon extract in the presence and absence of supplemented insulin and subsequently measured MCF-7 cells viability. In this study, we report a noble finding that bitter melon extract exerts cytotoxic effects on MCF-7 cells possibly via inhibition of glucose uptake. Our findings show that insulin rescues MCF-7 cells from the effects of bitter melon extract.
- Erklärung der Rechte:
- http://rightsstatements.org/vocab/InC/1.0/
- Lizenz:
- https://creativecommons.org/licenses/by/4.0/
- Sprache:
- English
- Herausgeber:
- California Institute of Technology
- Identifikator:
- DOi: 10.17912/micropub.biology.000961, PMCID: PMC10509689, and PMID: 37736248
- Ressourcentyp:
- Article
- Stichwort:
- bitter melon, metastatic breast cancer, MCF-7 cell, glucose clearance, supplemented insulin, and cytotoxic effects
- Datum erstellt:
- 2023